Effect of various hesperidin and naringin addition levels on specific sperm indicators and the integrity of their DNA as measured by the comet assay in aged broiler breeder males

Abstract

In broiler breeder males, the generation of semen decreases with age, and the body produces more free radicals when aging symptoms start to appear. Certain citrus wastes are a potentially sustainable source of high-biological value compounds with antioxidant properties that can enhance the performance of the male reproductive system. Hesperidin and naringin are two examples of these substances. Each treatment was given to the roosters separately, and their impacts on DNA integrity and semen parameters were later investigated using the comet assay. in contrast to the treatment used as the negative control. Most of the sperm characteristics (ejaculate volume, overall sperm motility, progressive sperm motility, sperm concentration, percentage of dead sperm, and percentage of abnormal sperm) were significantly and meaningfully improved by the hesperidin and naringin treatment. The percentage of genetic material that remains intact increased in the comet assay in favor of the treatments that also contained naringin and hesperidin. Finally, this study showed that hesperidin and naringin are sustainable substances led to improved semen qualities of elderly broiler breeder males and significantly increased the proportion of genetic material that was intact.