Abstract
Pathogen of Fusarium oxysporum f.sp. cubence (Foc) Vegetative Compatibility Group (VCG) 01213/16 (Tropical strain/TR4) and VCG 01218 (strain1) are the cause of wilt disease in banana. Plant-based pesticides are an alternative environmentally friendly substance that can control plant pathogens. This study aims to obtain a formulation of Piper aduncum oil with several essential oils to suppress the growth development of two Foc strains that cause wilt disease in banana plants. This study used a complete randomized design in factorial, where the first factor is the type of formulation: F1 = 25% P. aduncum leaf oil, F2 = P. aduncum leaf oil with lemongrass leaf oil (Cymbopogon flexiosus), F3 = P. aduncum oil with citronella leaves (Andropogon nardus), F4 = P. aduncum oil with Ceylon cinnamon leaf oil (Cinnamomum zeylanicum), F5 = P. aduncum oil with Padang cinnamon leaf oil (C. burmanii), F6 = P. aduncum oil with cloves leaf oil (Eugenia aromatica) F7=P. aduncum oil with wild ginger oil (Elettariopsis slahmong). The second factor is the concentration level: 1000, 2000, and 3000 ppm. The results showed that diameter of mycelium between Foc VCG01213/16 and VCG01218 did not differ significantly. However, there were significant differences in the botanical pesticide formulations used. P. aduncum oil formulation mixed with cloves leaf oil and ceylon cinnamon oil at a concentration level of 3000 ppm had better inhibition of colony diameter, namely for Foc VCG01213/16 (90.55% and 88.08%) and on Foc VCG 01218 (90.72% and 89.85%). Likewise, for the inhibition of colony biomass, the best formulation is the P. aduncum oil mixed with cloves leaf oil and with ceylon cinnamon oil, the concentration level at 3000 ppm for Foc VCG 01213/16 were 96.64 and 95.80%, respectively. In contrast, for Foc VCG 01218 were 96.00% for both.
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