Abstract
Water mint (Mentha aquatica) is well-known as a popular herbal plant in Viet Nam. Rosmarinic acid (RA) is a phenolic compound found in many genus of Mentha and has numerous biological activities. Its applications are wide from the food industry to cosmetics. The purpose of this study was to evaluate the effects of different plant parts, drying, various solvent to sample ratios (1:20, 1:30, 1:40, 1:50, 1:60, and 1:70 (w/v)) and extraction temperature (30, 50, 70, and 90°C) on RA content of water mint. Ground materials of fresh and dried leaves, fresh and dried whole plants (each for 1 g) were extracted with water. The extraction was performed on magnetic stirrer. The extract was passed through filter paper retaining the clear solution for the quantitative and RA analysis. RA content was determined by using a spectrophotometer. The dried whole plants, a solvent to sample ratio of 1:50 (w/v), extraction temperature of 70°C, and extraction time of 30 minutes were the best conditions for RA content from water mint.
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