EFFECT OF VARIOUS DRUGS ON THE DISTRIBUTION OF 65ZN IN EHRLICH ASCITES CANCER-BEARING MICE

Abstract

Various drugs (anti-cancer agents, chelating agents, amino acids and salts) were injected i.p. with 65Zn into Ehrlich ascites cancer-bearing mice, and after 1 hr its effect on the organ distribution of 65Zn was investigated.1. Mitomycin C (200 μgg/mouse), Toyomycin (50 μg/mouse) and Nitromin (500 μg/mouse) had no effects on the distribution of 65Zn. Merphyrin (500 μg/mouse) caused a decrease in 65Zn uptake by cancer cells without any effects in the other organs.2. EDTA and BAL (each 1 mg/mouse) inhibited 65Zn> uptake by cancer cells and conversely elevated its concentration in ascites supernatant. BAL elevated also 65Zn concentration in other various organs. Tetracycline, dihydrothioctic acid and Na-thioglycolic acid (each 1 mg/mouse) did not affect the 65Zn uptake by cancer cells.3. Penicillamine (D-and DL-type, each 1 mg/mouse) caused a decrease of 65Zn concentration in cancer cells with an increase of its concentration in the other organs. Cysteine, histidine and glycine (each 1 mg/mouse) had no effects on the 65Zn distribution in every organs.4. Various salts (each 0.1 ml of 3.5 ×10-2M solution was injected per mouse) also affected organ distribution of 65Zn. ZnSO4, CoCl2 and NiSO4 inhibited the uptake of 65Zn by cancer cells, however, MgCl2, MnSO4, FeCl<2 and CuSO4 had no effects on it. Organ distribution of 65Zn was also affected by various salts without definite relationship between them.5. In ascites cancer cells, the highest concentration of 65Zn was found in supernatant fraction, next in nuclear and microsomal fraction. The mitochondrial fraction contained the lowest amount. All of the above drugs had no effect on the intracellular distribution pattern of 65Zn in cancer cells.