Effect of uterine horn ligation on bone mass: an experimental study in rats

Abstract

Objective: After tubal ligation, normal bone mass in the presence of gonadal deficit has been reported. These incongruent results motivated us to examine the topic. Study design: Bone mass was assessed by densitometry and ultrasonography 60 days after surgery on 100-day-old female Wistar rats. Fifteen Wistar rats with uterine horn ligation (TL) were compared with 15 unoperated, 15 with a sham uterine horn ligation (Sham-TL), and 15 ovariectomized (OVX), using ANOVA and a correlation test to determine the relations between results. Results: Femoral and vertebral bone mass were significantly lower in the OVX y TL groups than in unoperated and controls groups ( P<0.0001). Conclusions: Our study revealed significantly lower axial and peripheral bone mass in rats with uterine horn ligation.