Effect of upregulation of Raf kinase inhibitory protein on the biological behavior of cervical cancer cells

Abstract

Objective Investigate the effect of up regulation of RKIP (Raf kinase inhibitory protein) gene on the biological behavior of cervical cancer cell line SiHa. Methods pcDNA3. 1(+ )-RKIP was transfected into cervical cancer SiHa cells by liposome. After stable screening, the cells were divided into the blank control, empty vector transfected, and RKIP transfected group. MTT assay was used to detect cell proliferation, adhesion, and invasion abilities in vitro. The cell cycle changes were detected by flow cytometry. Results The RT-PCR found that RKIP mRNA expressions in the control and empty vector groups had no changes while high expression of RKIP mRNA was found in transfected cells. The cell proliferation in transfected cells significantly decreased compared to that in the blank control and vector transfected group. The cells adhesion and invasion abilities in vitro significantly decreased in the transfected cells compared with the blank control and vector transfected group. The difference above was statistically significant (P=0.010, 0.040, 0.002, respectively). In transfected cells, the cells were blocked in G1 phase and the percentage of G2+ S phase cells was decreased, while in the control and empty vector groups, the cells showed the decreas in the percentage of cells in G1 phase but increase in the percentage of cells in G2+ S phase. Conclusion RKIP can significantly reduce the proliferation and invasion ability of cervical cancer cells, which may be the tumor suppressor gene of cervical cancer, and its possible mechanism is related to the change in cell cycle distribution. Key words: Cervical cancer; SiHa cell; RKIP; Transfection