Effect of Uncaria gambir and Uncaria sclerophylla on Pulmonary- Thromboembolism Mice

Aditya Sindu Sakti,Astari Rachma Nityasa,Fadlina Chany Saputri

doi:10.5530/pj.2020.12.29

Abstract

Background: Previous studies on virtual screening on P2Y12 receptor of Adenosine Diphosphate (ADP) have showed that Roxburghine B, the compound which is found in Uncaria species, can inhibit the receptor function. Objective: In this study, we investigated the effect of Uncaria gambir and Uncaria sclerophylla extract on survival rate and bleeding time as antithrombotic in vivo. Methods: Animal subjects (ddY strain mice) were divided to two different experimental group (survival rate and bleeding time). U. gambir and U. sclerophylla were given to the mice orally in three different dose (5 mg, 10 mg, 20 mg/20 g BW and 2.5 mg, 5 mg, 10 mg/20 g BW, respectively) for seven days. Results: U. gambir and U. sclerophylla able to prolong bleeding time from test subjects equivalent to ASA as standard. The results show the increasing number of survived animals in the treated group compared to the negative control group. Conclussion: Both of U. gambir and U. sclerophylla prevent pulmonary thromboembolism on mice subjects represent by the increased of survival rate. Antithrombotic effects that were observed suggested was provide by their antiplatelet activity.

Highlights

The third leading cause of death by cardiovascular disease after myocardial infarction (MI) and cerebrovascular accidents (CVA) is venous thromboembolism (VTE).[1]
The differences in mean (Table 1.) was analyzed with ANOVA test followed by Tukey and LSD post hoc analysis using SPSS v.22, values of p < 0.05 were considered to be statistically significant. This study revealed both of U. gambir and U. sclerophylla were proven able to prolong bleeding time from test subjects equivalent to acetylsalicylic acid (ASA) as standard
Pulmonary thromboembolism induction caused 60% of deaths from each group administrated with CE injection alone

Summary

Introduction

The third leading cause of death by cardiovascular disease after myocardial infarction (MI) and cerebrovascular accidents (CVA) is venous thromboembolism (VTE).[1]. Data reported the incidence of VTE annually ranges from 104-183 person cases per year, its similar the rate of stroke cases. Whereas the incidence of PE without DVT ranges from 29-78 cases per 100.000 person for each year.[5] this condition will impact on global health problem, reducing the quality of life and mortality of patients. Objective: In this study, we investigated the effect of Uncaria gambir and Uncaria sclerophylla extract on survival rate and bleeding time as antithrombotic in vivo. Methods: Animal subjects (ddY strain mice) were divided to two different experimental group (survival rate and bleeding time). Results: U. gambir and U. sclerophylla able to prolong bleeding time from test subjects equivalent to ASA as standard. Conclussion: Both of U. gambir and U. sclerophylla prevent pulmonary thromboembolism on mice subjects represent by the increased of survival rate.

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Discussion

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