Abstract
Ultrasound pre-treatment of intact hemp seeds without any solvent assistance was carried out for 10, 20 and 40min prior to SCCO2 extraction at 40°C, 300bar and 45kg CO2/kg feed. Sonication time effect on SC-CO2 extraction was investigated by the extraction kinetics. The maximum extraction yield was estimated to be 24.03 (% w/w) after 10min of ultrasonic pre-treatment. The fatty acid compositions of the oils extracted by SC-CO2 without and with ultrasound pre-treatments was analyzed using gas chromatography. It was shown that the content of linoleic, α-linolenic and oleic acids (the most abundant unsaturated fatty acids) of the hemp seed oils were not affected significantly by the application of ultrasound. UV spectroscopy indices (K232 and K268) and antiradical capacity were used to follow the quality of oils. Significant were the changes in their antiradical capacity due to ultrasound treatment. A comparison with the oil extracted by Soxhlet was also given.
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