Abstract
Dilute suspensions of canine erythrocytes were exposed to 1 Mc focused ultrasonic fields with maximum acoustic pressures estimated at 10 atm. In standing wave patterns in which circulation was inhibited, the cells clumped rapidly at the nodes and no changes were found. With a free surface, fountaining and stirring occurred. Exposure at 1 atm ambient pressure then resulted in rapid lysis. Erythrocytes exposed briefly in isotonic Rous-Turner mixture and controls were both diluted with sodium chloride solutions of varying concentration. The optical density at 650 mμ was plotted as a function of salt concentration. The exposed samples showed a greater fractional decrease in optical density at low sodium chloride concentrations than did the controls. Other samples exposed in hypotonic solutions showed both the greater sensitivity to low sodium chloride concentrations and a smaller change in optical density as the sodium chloride was varied from isotonic to 70% isotonic. It has not been possible to demonstrate that any factor other than cavitation was responsible for the observed changes in osmotic fragility.
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