Abstract
Background: Tissue engineering involves using different types of stem cells. One of the roadblocks in tissue engineering is the scant supply of stem cells. The potential use of human umbilical cord peri-vascular Cells (HUCPVCs) has recently been considered as an important cell source for tissue engineering applications. The objective of this study was to explore the effect of low intensity pulsed ultrasound (LIPUS) on HUCPVCs. Materials and methods: HUCPVCs were divided into two groups: treatment group which received 30 mW/cm2 LIPUS for 10 minutes (1, 7, and 14 days) and control group which received sham treatment. The study groups were evaluated for cell count, alkaline phosphatase (ALP) activity, DNA-content, gene expression, and immunophenotype. Results: There was no significant differences in cell count, ALP, DNA-content, and CD-90 between LIPUS and control groups. A significantly higher expression of OSP and PCNA was observed on day 14 in LIPUS treatment group. Conclusion: LIPUS application for 10 minutes per day for 14 days enhanced OSP and PCNA expression without significant increase in cell count of HUCPVCs. Future research may aim at exploring different LIPUS applications (different time and frequency) to optimize HUCPVCs proliferation.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.