Effect of Ultrasound Frequency and Treatment Duration on Antibiotic Elution from Polymethylmethacrylate Bone Cement

Abstract

The objective of this study was to evaluate the effect of ultrasound frequency and treatment duration on antibiotic-impregnated polymethylmethacrylate (PMMA) antibiotic elution rates and mechanical strength. Two batches of PMMA were prepared: one with five grams of vancomycin powder and one without. Each batch was divided into two frequency groups: kHz and MHz. Each frequency group was divided into two duration groups: two minutes and ten minutes. Elution samples were measured daily using flow injection analysis. After one week of elution, ultrasound treatments were done daily until each group's average concentration fell below those of non-ultrasound control groups. After elution testing, compression testing determined mechanical properties. Paired t-tests were used to compare daily elution amounts to baseline values. Univariate ANOVAs were used to test for effects of both frequency and treatment duration on antibiotic elution amounts and on mechanical properties. All ultrasound treatments resulted in significant increases in antibiotic elution. Frequency and duration had significant effects of increasing antibiotic elution (p < 0.001). The kHz group produced significantly greater antibiotic elution than the MHz group (p < 0.001). The 10-minute duration produced significantly greater antibiotic elution than the two-minute duration (both p < 0.001). Frequency and duration did not have significant effects on yield stress (p = 0.841 and p = 0.179, respectively). Frequency had a significant effect (p = 0.024) on modulus, but duration did not (p = 0.136). Ultrasound frequency and treatment duration significantly affect antibiotic elution from PMMA which may be helpful for treatment of periprosthetic joint infections during revision arthroplasty.