Abstract
In present work, the effects of ultrasound on activity and conformation of polyphenol oxidase (PPO) from potato were investigated under different ultrasonic gradient for ultrasonic power (240-600 W), time (15-120 min), temperature (20-50℃) and pH (5.4-8.2). The results indicated that potato PPO exhibited a decrease in activity treated by different ultrasonic treatment conditions. Furthermore, the ultrasound significantly down-regulated the expression of PPO gene. The CD analysis demonstrated that the secondary structures such as α-helix were destroyed compared with the pristine PPO. The PPO activity showed a corresponding decrease along with the changes in the secondary structure. This investigation revealed that ultrasound inactivate PPO activity by changing the conformation of enzyme protein and down-regulating gene expression.
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