Abstract
Although ultraviolet (UV) radiation is used to treat several types of diseases, including rickets, psoriasis, eczema, and jaundice, the prolonged exposure to its radiation may result in acute and chronic health effects particularly on the skin, eyes, and the immune system.Aim: This study was carried out to show the effect of UV on both of the lymphoproliferative response and their capacity to produce IL-12 and IL-10 in mice.Methods: Mice were exposed to whole body UVB and tested for the effect of recovery times on lymphocyte proliferation and cytokine production. In addition, direct irradiation of spleens and lymphocyte suspension was carried out. Basal and mitogens-stimulated lymphocyte proliferation was assessed by MTT assay while IL-10 and IL-12 were measured using ELISA.Results: There was a significant suppression in lymphocyte proliferation in comparison with control. IL-12 level was significantly reduced while the level of IL-10 was increased. Con A and PWM mitogens had no significant changes in IL-10 while Con A caused a highly significant increase in IL-12 at day 6 of recovery in UVB body irradiation.Conclusion: Exposure to UVB radiation could cause a state of immune suppression and shifts Th1/Th2 cell response. This effect is closely associated with the reduction of Th1 cytokines’ expression and increase in Th2 cytokines’ levels.
Highlights
Ultraviolet (UV) spectrum includes short-wave (UVC; 200–290 nm), mid-wave (UVB; 290– 320 nm), and long-wave (UVA; 320–400 nm) components of the radiation
There was no significant difference between concanavalin A (Con A)- and PWM-induced IL-10 or interleukin 12 (IL-12) release (p = 0.963 and 0.870, respectively)
Our study focused on the effect of different durations of recovery on orienting the immune modulation induced by exposure to UVB radiation
Summary
Ultraviolet (UV) spectrum includes short-wave (UVC; 200–290 nm), mid-wave (UVB; 290– 320 nm), and long-wave (UVA; 320–400 nm) components of the radiation. UVB radiation is proved to be a mutagen, and has been shown to be responsible for sunburn, oxidative stress, and immune suppression. Decreases in the stratospheric ozone layer are permitting more UVB radiation to reach the Earth’s surface (Katiyar, 2007). Photoimmunology is a term defined as the study of the effects of non-ionizing radiation on the immune system. It grew from experiments designed to understand the mechanism(s) underlying UVB-induced skin carcinogenesis (Ullrich and Byrne, 2012). Numerous studies in the field of Photoimmunology have tried to identify the biological impact of UV on the mammalian immune
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