Abstract
The present study aimed at evaluating the effect of two strains of probiotics (Bacillus subtilis PB6 and Bacillus Cereus var. toyoi) on selected indicators of the antioxidant status and immune responses of broilers before and after challenge with Salmonella enterica subsp. Typhimurium (S. typhimurium). Birds were distributed into the following five treatments: negative control (CTL−); negative control+S. typhimurium (CTL+); antibiotic+S. typhimurium, (AB); Toyocerin(r)+S. typhimurium (TOYO); and CloSTATTM+ S. typhimurium (CLOS). Birds in all treatments, except for CTL−, were orally challenged with 3 x109 CFUS. typhimurium at 16 days of age. The results showed that total antioxidant capacity (TAC), superoxide dismutase activity (SOD), and hydrogen peroxide (H2O2) values were similar among all groups. The antibody titers against S. typhimurium and Newcastle disease (ND) were not affected by the treatments (p>0.05). However, S. typhimurium and ND titers were influenced by the interaction between week and treatment (p<0.01, p<0.05, respectively) and by week (p<0.001, p<0.001, respectively). In conclusion, S. typhimurium challenge had an impact on anti-Salmonella and NC titers. Both probiotics did not affect the broilers' antioxidant capacity.
Highlights
Salmonella enterica serovar Typhimurium (S. typhimurium) is an enteric bacterium that can colonize chickens; it is a common serovar causing salmonellosis in broiler chickens (Ribeiro et al, 2007).Probiotics have been reported to prevent the colonization of the gut by pathogenic bacteria like Clostridium perfringens and Salmonella through the mechanism of competitive exclusion (Teo and Tan, 2007)
Serum TAC, superoxide dismutase activity (SOD), and H2O2 values were similar (p>0.05) among all groups, suggesting that neither S. typhimurium challenge nor probiotics had an impact on total antioxidant capacity or oxidative stress
These results agree with Erdoğan et al (2010),who concluded that the probiotics tested did not promote any significant improvement in the oxidant/antioxidant status in broilers
Summary
Probiotics have been reported to prevent the colonization of the gut by pathogenic bacteria like Clostridium perfringens and Salmonella through the mechanism of competitive exclusion (Teo and Tan, 2007). A Bacillus subtilis-based probiotic was capable of producing an antimicrobial factor against many bacteria (Yurong et al, 2005). Other reports showed that a probiotic based on Bacillus Cereus var. Probiotic supplementation was shown to modulate the dynamics of oxidants and antioxidants in the body. Probiotics have been shown to increase antioxidant levels and reduce glutathione concentrations and intestinal oxidative injury (Yadav et al, 2007). Sohail et al (2011) demonstrated that a probiotic reduced the total antioxidant concentration. Rats fed a probiotic showed increased SOD and glutathione peroxidase concentrations (D’Souza et al, 2010)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
