Abstract
The effectiveness of applying Ovaprim [(D-Arg6, Pro9NEt)-sGnRH + domperidone] and Ovopel [(D-Ala6, Pro9NEt)-mGnRH + metoclopramide] to male barbel Barbus barbus (L.) 6, 12 and 24 h after hormonal stimulation was analyzed. The control group (Control) during each time interval was stimulated with 0.9 % NaCl. Milt was collected from seven fish only once (n = 7) for Ovopel, Ovaprim and Control group in order to determine total volume of milt, volume of milt per kg of body weight, sperm concentration, total sperm production, seminal plasma osmotic pressure, pH of milt and pH of seminal plasma. Woynarovich’s solution (68 mM NaCl + 50 mM urea) with the addition of 0.5 % BSA (pH 7.7; 181 mOsm kg−1) was used as the activating liquid. Selected parameters of sperm motility (MOT %) and progressively motile sperm (%), curvilinear velocity (VCL, μm s−1), straight-line velocity (μm s−1), movement linearity (%), wobbling index (%), amplitude of lateral head displacement (μm) and beat cross frequency (Hz) were determined using the Computer-assisted sperm analysis system. A time of 6 h proved to be too short to obtain milt from barbel following hormonal stimulation with Ovaprim and Ovopel. Extending the time to 12 h, however, resulted in 100 % spermiation in males, regardless of hormonal preparation used for stimulation. The stimulation of spermiation in barbel is best performed using Ovopel 12 h upon application. Extending the latency period to 24 h following the application of this preparation results in a significant decrease in the volume of milt obtained, sperm count and motility parameters, including MOT and VCL, which may influence sperm fertilization ability.
Highlights
Conservation aquaculture is a very important tool for the restitution and support of fish species which are in danger of extinction (Philippart 1995; Ross et al 2008)
After 12 h, milt was obtained from 42.86 % (n = 3) of barbels from the control group and 100 % of barbels stimulated with Ovaprim and Ovopel
The pH of seminal plasma correlated negatively with sperm concentration in the Ovaprim group (Table 5). This is the first study to show that spermiation stimulation of barbel can be conducted using a gonadoliberin analogue other than Ovopel, that is Ovaprim, and milt collection after Ovaprim application at a temperature of 19–20 °C can be extended to 24 h without quality losses
Summary
Conservation aquaculture is a very important tool for the restitution and support of fish species which are in danger of extinction (Philippart 1995; Ross et al 2008). Over the recent years, there has been increased interest in research aimed at devising effective procedures for producing high quality larvae and fry fish of endangered species (Targonska et al 2010; Z_ arski et al 2011). The quality of gametes under controlled conditions is determined by the preparation used for hormonal stimulation, which is necessary for obtaining gametes of sufficient quantity and quality (Rurangwa et al 2004; Kowalski et al 2012). The existing research has confirmed a high efficiency of ovulation and economic profitability of artificial spawning among barbel bred in captivity under controlled conditions following the application of Ovopel and Ovaprim (Hakuc-Błaz_owska et al 2010; Targonska et al 2011)
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