Effect of Two Amino Acid Solutions on Leucine Turnover in Preterm Infants

Abstract

Objective: To assess the effect of two different parenteral amino acid mixtures, Trophamine^® and Primene^®, on leucine turnover in preterm infants. Method: Leucine kinetics were measured with [5,5,5 D3]leucine tracer in 15 infants receiving Trophamine (group ‘T’) (mean birth weight 1,263 g) and 22 who received Primene (group ‘P’) (mean birth weight 1,336 g) during two study periods, within a few hours after birth but before introduction of parenteral amino acid solution, and again at postnatal day 7. The rate of appearance of leucine was calculated from the enrichment of α-ketoisocaproic acid in plasma. Results: There were no significant differences in leucine turnover within a few hours after birth in the two groups. In the infants who received Primene leucine turnover on day 7 was significantly lower than in those who received Trophamine (269 ± 43 vs. 335 ± 27, p < 0.05). Despite a higher intake of leucine in the Trophamine group (108 ± 10 vs. 77 ± 8 µmol·kg^–1·h^–1), leucine released from proteins at day 7 was higher in this group compared to Primene (227 ± 27 vs. 192 ± 42 µmol·kg^–1·h^–1). Conclusions: Primene administration results in lower leucine released from proteins, an estimate of protein breakdown, than Trophamine in preterm infants. Increases in whole body leucine turnover in response to administration of i.v. amino acids is influenced by the composition of the amino acid mixture. The factors responsible for this difference remain to be elucidated.