Abstract
Purpose: To investigate the effect of dimethyl sulfoxide (DMSO) and water extracts of Turkish propolis (WEP) on mRNA expression of Nav 1.5 and 1.7 α isoforms of Voltage-Gated Sodium Channel (VGSC) proteins in PC-3 human prostate cancer cells. Methods: DMSO and WEP (20 μg/mL each) were incubated for 24 h with PC-3 cells and total RNA was extracted using a commercial kit. Real-time polymerase chain reaction (RTPCR) assay was used to determine mRNA levels of the isoforms of VGSC. Expressions of VGSC were assumed to be 100 % in PC-3 cells incubated without extract. Results: Both extracts decreased the expression of VGSC isoforms to varying extents. Expressions of Nav 1.5 and 1.7 was 61.43 ± 4.92 and 58.17 ± 2.88 %, respectively for DMSO; while for WEP, the values were 83.54 ± 15.96 and 80.40 ± 13.87 20 μg/mL, respectively. Conclusion: This results suggest that DMSO and water extracts of Turkish propolis may have antimetastatic activity in PC-3 cells due to down-regulation of expressions mRNA of VGSC α-isoforms. Keywords: Propolis, Voltage-gated sodium channel (VGSC), PC-3 Human prostate cancer cells
Highlights
Propolis is a bee product which has been used in folk medicine since ancient times [1]
We investigated the effects of propolis extracts on mRNA expression of Voltage-Gated Sodium Channel (VGSC) Nav 1.5 and Nav 1.7 α-isoforms in PC-3 cells
0.5 mL of 20 μg/mL water extracts of Turkish propolis (WEP), 20 μg/ml dimethyl sulfoxide (DMSO) extract of Turkish propolis and 1 ml of FBS were added to 8.5 mL of RPMI1640 and the cancer cells were incubated for 24 h at 37 oC and 5 % CO2 after adding the new medium mixture
Summary
Propolis is a bee product which has been used in folk medicine since ancient times [1]. We investigated the effects of propolis extracts on mRNA expression of VGSC Nav 1.5 and Nav 1.7 α-isoforms in PC-3 cells. 0.5 mL of 20 μg/mL WEP, 20 μg/ml DMSO extract of Turkish propolis and 1 ml of FBS were added to 8.5 mL of RPMI1640 and the cancer cells were incubated for 24 h at 37 oC and 5 % CO2 after adding the new medium mixture. CDNA was synthesized from 5 μL of total RNA in a final volume of 20 μL reaction using the AMV(Avian Myeloblastosis Virus) Reverse Transcriptase (Roche Diagnostics, GmbH, Mannhein, Germany) with Nav 1.5, Nav 1.7 and β-Actin primers at 2.5 μL from 10 pmol following the manufacturer’s protocol. All data were expressed as arithmetic mean ± standard deviation (SD)
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