Effect of tumor suppressor gene phosphatase and tensin homologue deleted on chromosome ten on biological behavior of human thyroid papillary carcinoma cell line

Abstract

Objective To observe the effect of phosphatase and tensin homologue deleted on chromosome ten (PTEN) gene expression on the biological behavior of human thyroid papillary carcinoma cell line (TPC-1). Methods Transfection of PTEN overexpression vector (pBP-PTEN) in human TPC-1 cell line, Simultaneous transfection of empty vector (pBP) as a control, Western blotting assay for expression of PTEN in human TPC-1 cell line, the effect of PTEN on cell proliferation of TPC-1 cell line was observed by measuring the absorbance A value in the thiazolyl blue colorimetric (MTT) assay, transwell chamber to observe the effect of PTEN on cell invasion and migration of TPC-1 cell line by detecting the number of cells, the collected values are expressed as mean±standard deviation (Mean±SD), and t test is applied. Results In the western map, the gray level of the over-expressed vector group showed that the expression level of PTEN in TPC-1 cells was significantly higher than that in the empty vector control group, the proliferation ability, cell invasion and migration ability of TPC-1 cells in the overexpression vector group [absorbance (A) value: 0.40±0.02, invasive cell number: (45.76±4.11) cells, migration cell number: (25.62±2.89) cells] were significantly lower than the empty vector control group (A value: 0.75±0.03, the number of invasive cells was (95.32±5.41) cells, and the number of migrated cells was (53.47±4.42) cells, the difference was statistically significant (P<0.05). Conclusion Overexpression of PTEN can significantly inhibit the proliferation, invasion and migration of thyroid papillary carcinoma cells. Key words: Phosphatase and tensin homologue deleted on chromosome ten; Thyroid papillary carcinoma; Cell proliferation; Tumor invasion; Tumor migration