Effect of tulsi extract and honey mouthrinses on salivary Streptococcus mutans count in comparison with 0.2% of chlorhexidine: A randomized controlled trial

Abstract

Introduction: Mouthrinses have been popularly used as a supplementary oral hygiene aid. A lot of commercially available mouthrinses possess few adverse effects, which has necessitated the search for alternative and herbal mouthrinses. Aim: This study aims to evaluate the effect of tulsi extract and honey mouthrinses on Streptococcus mutans count in comparison with 0.2% of chlorhexidine. Materials and Methods: A randomized controlled trial was conducted in various schools of Nellore District, Andhra Pradesh. A total of forty-five individuals aged 15 years who satisfied the inclusion criteria were randomly allocated to three group, i.e., 15 each for Group A (0.2% of chlorhexidine mouthrinse), Group B (honey mouthrinse), and Group C (tulsi extract mouthrinse) by lottery methods. Oral hygiene status was assessed and evaluation of S. mutans was done before and after giving the mouthrinse. The sample was streaked on mitis salivarious-bacitracin agar medium. Results: There was a reduction in S. mutans in all the three groups with 0.2% of chlorhexidine showing a maximum reduction. There was significant difference between Group A (0.2% chlorhexidine mouthrinse) and Group B (honey mouthrinse) and between Group A (0.2% chlorhexidine mouthrinse) and Group C (tulsi extract mouthrinse) (P < 0.001). There was no significant difference between Group B (honey mouthrinse) and Group C (tulsi extract mouthrinse) (P = 1.00) in S. mutans count. Conclusion: Herbal mouthrinses containing tulsi and honey, though as not as effective as chlorhexidine in its antimicrobial property, have its own value and it can be effectively used in areas where people cannot access to chlorhexidine.