Effect of Tualang honey on the anastomotic wound healing in large bowel anastomosis in rats-A randomized controlled trial.

Muhammad Izani Aznan,Syed Hassan Syed Abd. Aziz,Sharifah Emilia Tuan Sharif,Omaid Hayat Khan,Amer Hayat Khan,Allah Obhayo Unar,Andee Dzulkarnaen Zakaria

doi:10.1186/s12906-016-1003-6

Muhammad Izani Aznan, Syed Hassan Syed Abd. Aziz + Show 5 more

Open Access

https://doi.org/10.1186/s12906-016-1003-6

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Abstract

BackgroundHoney has long been used for the treatment of number of ailments and diseases including surgical wounds. Current study evaluates the effectiveness of Tualang honey (TH) for large bowel anastomotic healing in Wistar rats.MethodsThirty male Wistar rats were given a 3 centimeter infra-umbilical laparotomy wound, in`flicted on their abdomen. The colonic transection was performed at 5 cm distal to caecum, with end to end anastomosis of colon segment. They were divided into two groups. Group I was fed with standard rat chow and water. Meanwhile, Group II apart from standard feed, was also given TH 1.0 g/kg every morning until day seven post operatively. Afterwards, anastomotic bursting pressures were measured and histopathological examination on the anastomosis line was performed with light microscopes. The data from two groups were analyzed by Independent paired t test for continuous variables.ResultsIt was found that the tensile strength of colon anastomosis (95 % CI; p = <0.001) and the histopathological study including fibroblast count (p = <0.001) and inflammatory cells (p = 0.002) showed statistically significant difference in the favor of TH-treated group. Meanwhile, neovascularization formation was not statistically significant (p = 0.807); however, the overall count in the TH group was high.ConclusionOral treatment with TH enhances anastomotic wound healing by increasing the number of fibroblasts and by decreasing inflammatory cells leading towards increased wound strength.

Highlights

Honey has long been used for the treatment of number of ailments and diseases including surgical wounds
A disastrous complication of intestinal anastomosis is anastomotic leak resulting in peritonitis, which is associated with high mortality, morbidity and increased hospital stay [1]
The aim of the present study is to investigate the effect of Tualang honey (TH) in large bowel anastomosis in rats, undertaking its effect on tensile strength of large bowel anastomosis by measuring the bursting pressure and its effect on histopathology examination

Summary

Introduction

Honey has long been used for the treatment of number of ailments and diseases including surgical wounds. Current study evaluates the effectiveness of Tualang honey (TH) for large bowel anastomotic healing in Wistar rats. Since the very beginning of 17th century, intestinal anastomosis procedure is being practiced and has been considered a promising surgical intervention for patients with diseased bowels. A disastrous complication of intestinal anastomosis is anastomotic leak resulting in peritonitis, which is associated with high mortality, morbidity and increased hospital stay [1]. Apart from modern surgical techniques and precautions, intra-abdominal adhesion formation after surgery is still an obvious manifestation [2]. The incidence of anastomotic leak varies widely with a usual range between 2.7 % to 26 % in abdominal surgeries [1, 5], risk factors for which normally remain type of procedure, patient demographics and surgical techniques.

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