Abstract
Objective: The goal of regenerative endodontic therapy is biological healing of the pulp tissue. It involves the disinfection of the canals with irrigantsand medicaments. The medicaments that are currently used for this purpose are a triple antibiotic paste (TAP), calcium hydroxide (Ca[OH]2), andLedermix®, a paste containing demeclocycline and triamcinolone. Therefore, the purpose of this study was to evaluate the effects of TAP, Ca(OH)2, andLedermix® on the viability of dental pulp stem cells (DPSC).Methods: Primary cultures of DPSC were obtained from immature third molars. Immunofluorescence assay using STRO-1 marker was performedto confirm the mesenchymal nature of the DPSC. The cells were exposed to TAP, Ca(OH)2, and Ledermix® at concentrations of 0.1 and 1 mg/mL. Cellviability was analyzed using the MTT assay.Results: Significant differences in viability were noted between the cells exposed to the medicaments and those in the control group (p<0.05).Conclusions: All three medicaments decreased the viability of DPSC, with the Ledermix® paste demonstrating the highest toxic effect.
Highlights
The ultimate goal of endodontic treatment is the achievement of biological healing in the form of regeneration
All three medicaments decreased the viability of Dental pulp stem cells (DPSC), with the Ledermix® paste demonstrating the highest toxic effect
Very few studies on endodontic regeneration have been conducted in Indonesia; this study aimed to evaluate the effects of root canal medicaments commonly used in root canal treatment on the viability of mesenchymal stem cells
Summary
The ultimate goal of endodontic treatment is the achievement of biological healing in the form of regeneration. Network regeneration is the process of healing by restoring the natural architecture and biological functions of damaged tissue, whereas repair is the restoration of tissue with replacement or scar tissue, without the return of function [1]. The conventional endodontic treatment procedures performed currently replace damaged tissue with biocompatible synthetic materials, but do not restore the biological functions to resemble that of normal tissues [2]. Dental pulp stem cells (DPSC) have a high rate of proliferation even after many subcultures. DPSC have the ability to modify due to its high rate of differentiation. The characteristics and multiline differentiation potential of DPSC make them an important part of regenerative care [4]
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