Effect of trim28 (ser473) phosphorylation on viability of hepatocellular carcinoma cells during DNA damage

Abstract

Objective To observe effect of Trim28 (ser473) phosphorylation on viability of hepatocellular carcinoma (HCC) cells during DNA damage. Methods To divide Hepg2 cells into 2 groups as SB203580+ UV group and UV group. Hepg2 cells were modeled for DNA damage by UV (Ultraviolet) irradiation. SB203580+ UV group was treated with Trim28 phosphorylation inhibitor SB203580 after UV irradiation. Expression of Trim28 and phosphorylation-Trim28 ser473 (P-Trim28 ser473)were detected by westernblot experiment. The viability of Hepg2 cells were observed by methyl thiazol tetrazolium (MTT) experiment. Results Compared with UV group (0.94±0.13), the expression value of Trim28 in SB203580+ UV group had no significant difference (t=-0.190, P>0.05). Value of phosphorylation-Trim28 in SB203580+ UV group (0.32±0.04) was obviously lower than UV group (0.72±0.05), the difference had statistical meaning (t=8.500, P<0.05); The 24, 48 and 72 hours after UV irradiation, the cell survive rate of UV group [(95±7)%, (62±10)%, (35±6)%] is higher than SB203580+ UV group [(85±7)%, (37±4)%, (20±3)%]. The difference has statistical meaning (t=-2.326, -5.212, -4.577, P<0.05). Conclusion The viability of HCC cells during DNA damaged can be reduced by inhibition for Trim28 phosphorylation. Key words: Trim28; Phosphorylation; Cell survival