Effect of Tridax procumbens extracts on coagulation time according to gender and blood groups A and O: in vitro study

Abstract

IntroductionTridax procumbens (T. procumbens) is an Ayurvedic herb that has been used as an anticancer, antioxidant, and coagulant or anticoagulant drug due to its phytochemical components. The current study is designed to investigate the effect of the ethanolic extract of T. procumbens on prothrombin time for blood groups A+, A−, O+, and O− and for different genders. MethodsUsing the Lee-White method, various concentrations of T. procumbens extracts (50, 100, 200, 400, and 800 μg) were utilised to investigate how they affected the clotting time (CT). Twenty-four healthy volunteers (females and males) were involved, in which A+ and O+ blood groups were predominant. ResultsEthanolic T. procumbens extracts significantly and dramatically accelerate the clotting process for all tested blood groups. This effect was observed as a dose-dependent. The strongest coagulant activity was observed at 200 µg and 400 µg doses for individuals with the A blood group, as prothrombin time is significantly decreased. At concentrations of 200 µg and 400 µg, the CT is found to be 1.62, 2.4, 2.4, and 8.6 fold greater for O+, O−, A+, and A−, respectively, compared with normal blood samples. A notable reduction in the CT is observed in those with blood group A−, followed by A+. Meanwhile, the longest time is recorded for the O blood groups. ConclusionThe haemostatic activity of T. procumbens may be attributed to its phytochemical components. Furthermore, T. procumbens shows a reduction in clotting time among males as compared with females.