Effect of tricarboxylic acid cycle intermediates on acetic acid production by acetobacter aceti.

Abstract

The stimulative effect of tricarboxylic acid (TCA) cycle intermediates on the acetic acid production of Acetobacter aceti M23 was investigated in batch and continuous cultures. Cell growth was hardly affected by the supplement of the TCA cycle intermediates; however, the acetic acid production rate in a continuous culture at the dilution rate of 0.097h-1 increased 3.2 times in the medium supplemented with 2mM citric acid. The optimum concentration of citric acid as an additive in the medium was 10mM. Acetic acid production was enhanced by adding citric acid only when there was an insufficient supply of oxygen.