Abstract
Introduction This study was to investigate the effects of tricalcium silicate (Ca 3SiO 5) on proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) in vitro. Methods The hDPCs were seeded in culture medium with or without Ca 3SiO 5 extract and calcium hydroxide (Ca(OH) 2) extract. Proliferation of the hDPCs was measured by methyl-thiazol-tetrazolium (MTT) assay. Odontogenic differentiation of hDPCs was evaluated by real-time polymerase chain reaction by using odontogenic marker genes such as dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP 1), osteocalcin (OC), alkaline phosphatase (ALP), and collagen type I (Col I), which were verified by ALP activity assessment, mineralization assay, and immunocytochemistry staining for dentin sialoprotein (DSP). Results The MTT assay showed that hDPCs cultured with Ca 3SiO 5 extract proliferated more significantly as compared with Ca(OH) 2 extract. Analysis of odontogenic marker genes indicated that Ca 3SiO 5 enhanced the expression of those genes. Moreover, the extract of Ca 3SiO 5 stimulated mineralization and increased ALP and DSP production conspicuously. Conclusions These results reveal that Ca 3SiO 5 can induce the proliferation and odontogenic differentiation of hDPCs in vitro and might be a potential candidate for preparation of a new type of Ca 3SiO 5−based cement as a pulp-capping agent.
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