Effect of Treatment with Peptide Extract from Beef Myofibrillar Protein on Oxidative Stress in the Brains of Spontaneously Hypertensive Rats.

Lee Lee,Hur Hur

doi:10.3390/foods8100455

Abstract

This study was conducted to determine the effect of beef peptide extract on oxidative stress in the brains of spontaneously hypertensive rats (SHRs). A 3-kDa peptide extract was obtained from beef myofibrillar protein using alkaline-AK (AK3K). Oxidative stress in SHR brains was measured by assessing malondialdehyde (MDA) and reactive oxygen species (ROS) concentrations and superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) activity. The SHR brains treated with the AK3K peptide extract (400 mg/kg body weight, AK3K400) showed a significant decrease in MDA and ROS contents by 0.33 and 23.92 μM, respectively (p < 0.05) compared to the control. The SOD activity for AK3K400 was 61.26%, around 20% higher than the control. Furthermore, the SHRs treated with the AK3K peptide extract showed results similar to those obtained using captopril, a hypertension drug, except for the MDA level. The study demonstrates that the beef peptide extract inhibits the generation of oxidative stress in the SHR brain and could possibly be used for neuronal hypertension therapy.

Highlights

Hypertension is associated with increased oxidative stress in different organs such as the vascular tissue, brain, and kidney [1,2]
This study showed that the peptide extract from beef myofibrillar protein obtained using alkaline-AK has an anti-oxidative stress effect related to hypertension in the spontaneously hypertensive rats (SHRs) brain
The SHRs treated with AK3K peptide extracts showed a significant decrease in the MDA level and reactive oxygen species (ROS)

Summary

Introduction

Hypertension is associated with increased oxidative stress in different organs such as the vascular tissue, brain, and kidney [1,2]. Oxidative stress leading to the production of ROS is considered one of the risk factors for the development of cardiovascular disease, including hypertension, heart failure, and stroke [7,8,9]. Because oxidative stress is closely related to hypertension, decreasing oxidative damage may lead to a reduction in blood pressure and vasodilation [10]. Oxidative stress factors such as lipid peroxide, ROS, and superoxide dismutase (SOD) have been investigated in hypertensive patients and animal studies [11,12]. A previous study showed long-lasting activity of ACE inhibitors as hypertension agents in spontaneous hypertensive rat (SHR) brains [17]

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