Effect of transection at different levels of hypothalamus on the hypothalamo-hypophysial system of the toad, Bufo bufo, with particular reference to the ultrastructure of the zona externa of the median eminence.

Abstract

The ultrastructural changes taking place in the median eminence of Bufo bufo 3 hours to 4 months after transection of the brain at different levels, are described. 5 types of neurons in the zona externa of the median eminence of normal toads are described. All types of neurons degenerate, and profound changes of the ultrastructure of the capillaries are observed after transection just behind, or immediately in front of the optic chiasma. A few neurons containing dense granules with a diameter of about 1,000–1,300 A remain intact, however. The degeneration following denervation in front of the optic chiasma was considerably delayed compared to degeneration after denervation behind the optic chiasma. After transection more rostral to the optic chiasma, no significant degeneration of the median eminence was observed. The results are discussed with regard to degenerative dynamics and origin of the different nerve types. It is concluded, that all types of neurons terminating in the median eminence, originate at a level between the caudal and rostral parts of the preoptic nucleus, some fibres, however, containing dense, 1,000–1,300 A granules, originate caudally to the optic chiasma, in the posterior hypothalamus.