Abstract
目的 活体观察0.5%曲尼司特滴眼液对兔眼Tenon囊组织α2(Ⅰ)链前胶原及转化生长因子-β1(TGF-β1)mRNA表达的影响.方法15只新西兰兔随机分为3个组:实验组(应用0.5%曲尼司特滴眼液)、溶媒对照组(应用基础溶液)、正常对照组.采用半定量逆转录聚合酶链反应(RT-PCR)观察曲尼司特滴眼液对兔眼Tenon囊组织α2(Ⅰ)链前胶原及TGF-β1mRNA表达的影响.结果实验组、溶媒对照组、正常对照组的α2(Ⅰ)链前胶原/β-actin象素值比值分别为0.880±0.029、1.034±0.065、0.808±0.041,各组比值差异有显著统计学意义(q=4.808,3.207,6.551,P＜0.05).3个组的TGF-β1/G3PDH象素值比值分别为0.894±0.058、0.882±0.090、0.852±0.061,实验组与溶媒对照组的比值虽高于正常对照组,但各组差异无显著统计学意义(F=0.463,P＞0.05).结论0.5%曲尼司特滴眼液能明显抑制兔眼Tenon囊组织α2(Ⅰ)链前胶原mRNA的表达,可作为抗纤维化药物用于青光眼非穿透性小梁切除术后。
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