Effect of training on insulin secretion from single pancreatic beta cells

Abstract

The objective of this study was to determine whether insulin secretion from single pancreatic beta cells is reduced by endurance training. Male Sprague Dawley rats either trained (T, N = 9) for 11 wk on a rodent treadmill, remained sedentary, and were fed ad libitum (S, N = 8) or remained sedentary and were food restricted (pair fed, PF, N = 8) so that final body weights were similar to T. After training, T had significantly higher red gastrocnemius muscle citrate synthase activity compared with S and PF. In vivo insulin secretion was lower in T (4.6 +/- 1.4 ng.ml-1, mean +/- SEM of 70' + 90' concentrations during a hyperglycemic glucose clamp) when compared with S, 8.1 +/- 1.6 and PF, 9.7 +/- 1.7 ng.ml-1. In vitro insulin secretion from single beta cells was measured using the cell blot assay (Kendall and Hymer, Endocrinology, 121:2260-2262, 1987) and T, had lower secretion 2.6 +/- 1.4 pg.cell-1 when compared (P less than 0.05) with S, 6.7 +/- 0.6, but not lower than PF, 4.1 +/- 1.7 pg.cell-1. These data suggest that some of the training-induced reduction in insulin secretory response to glucose may be attributable to changes within the beta cell itself.