Effect of Topical Gel Administration of Secretome Hypoxia Mesenchymal Stem Cells (SH-MSCs) on IL-10 and TNF- α Gene Expression (In Vivo Experimental Study in Male Rats of Wistar Strains Model of Fluconazole-Induced Alopecia)

Abstract

Alopecia is a dermatological disorder characterized by disturbances in the shorter anagen phase and longer telogen phase in the hair cycle. Therapy with irritant side effects and contact dermatitis, scalp allergies. causes increased hair loss. Alternative therapy using secretome hypoxia mesenchymal stem cells (SH-MSCs) which is safe and effective is an option. Objective to determine the effect of topical administration of SH-MSCs gel on IL-10 and TNF-α gene expression in Wistar rats with a fluconazole-induced alopecia-like model. In vivo experimental research using a Post Test Only Control Group Design research design. This study used 4 groups, namely 2 treatment groups and intervention with a topical SH-MSCs gel dose of 20 μL and a dose of 40 μL, 1 treatment group that did not receive intervention (base gel control) and 1 group of healthy mice. Skin tissue analysis was carried out on day 22 to assess IL-10 and TNF-α gene expression using the RT-PCR method. IL-10 gene expression using the One way Anova test obtained a value of 0.00 (p<0.05) so that there was a significant difference in IL-10 gene expression between groups in mice with the alopecia like model. The results of the TNF-α gene expression data with a value of 0.00 (p<0.05) showed significant differences between treatment groups. Administration of various doses of SH-MSCs topical gel increased IL-10 gene expression and decreased TNF-α gene expression in Wistar rats with a fluconazole-induced alopecia-like model, with the use of SH-MSCs topical gel at a dose of 40 μL having the greatest effect. most significant compared to other groups.