Effect of topic nimodipine irrigation on cerebral perfusion after SAH

Abstract

Objective Base on confirmed efficacy to reduce pathological elevation of arterial blood velocity in SAH model, aiming to explore whether nirnodipine irrigation into subarachnoid space in SAIl model can indeed ameliorate abnormal perfusion status due to delayed vasosposm. Method 14 male minipigs were arranged into Sham, SAH or SAH + nimodipine groups randomly. SAH was manipulated with 5 ml autologous blood from femoral artery which was injected into its suprasellae cistern after craniotomy.40 ml Nimodipine dilution(O.04 mg/ml) was used to irrigate subaraehnoid space for SAIl + nmodipine group at the fourth days after model construction. Thirty minutes after irrigation, CT/SPECCT scans were acquired and the images were spatially normalized to template for perfusion analysis after reconstruction and realignment. ROI had been delineated on the CT template then register them onto corresponding normalized SPECT images, for CCR and RD calculation to senti-quantity hemisphere CBF and perfusion heterogeneity. Results Hemisphere hypoperfusion and more heterogeneous perfusion due to SAH were confirmed by CCR(1.988±0. 346 versus 1.382 ±0. 192, P 0. 05) and RD(0. 425 ±0. 018 versus 0.417 ±0. 015, P > 0.05) comparison between SAH + nimodipine and SAH group. Conclusions Nimodipine irrigation over intracranial arteries of SAH pigs can not improve cerebral perfusion abnormality. Key words: Subarachnoid hemorrhage; Models animal; Nimodipine; Vasospasm, intracranial; Cerebral blood flow