Abstract
The measurement of low concentrations of fluorescent tracers in sections of tissue is theoretically possible using several microfluorimetric techniques but the sensitivity attainable can be greatly decreased if high levels of autofluorescence are emitted by tissue components. The effects of tissue-processing techniques on autofluorescence and tracer fluorescence intensities were investigated. Autofluorescence in sections of rabbit carotid artery and fluorescence from lissamine rhodamine B-labelled albumin in a model system were measured by densitometry. Autofluorescence intensities appeared to be related to the degree of cross-linking resulting from fixation of the tissue. Tissue fixed with formaldehyde or mercury (II) chloride had the lowest emission. Fluorescence from the labelled albumin was not quenched by prolonged fixation with mercury (II) chloride and formaldehyde but extracting the “mercury pigment” from sections with alcoholic iodine resulted in a 34% decrease in intensity.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
