Abstract
Tissue factor (TF) is expressed abnormally in certain types of tumor cells, closely related to invasion and metastasis. The aim of this study was to construct a human gastric cancer cell line SGC7901 stably-transfected with human TF, and observe effects on oxaliplatin-dependent inhibition of invasion and the apoptosis induction. The target gene TF was obtained from human placenta by nested PCR and introduced into the human gastric cell line SGC7901 through transfection mediated by lipofectamine. Stably-transfected cells were screened using G418. Examples successfully transfected with TF-pcDNA3 recombinant (experimental group), and empty vector pcDNA3 (control group) were incubated with oxaliplatin. Transwell chambers were used to show change in invasive ability. Caspase-3 activity was detected using a colorimetric method and annexin-V/PI double- staining was applied to detect apoptosis. We generated the human gastric cancer cell line SGC7901/TF successfully, expressing TF stably and efficiently. Compared with the control group, invasion increased, whereas caspase-3 activity and apoptosis rate were decreased in the experimental group. TF can enhance the invasive capacity of gastric cancer cells in vitro. Its increased expression may reduce invasion inhibition and apoptosis-inducing effects of oxaliplatin and therefore may warrant targeting for improved chemotherapy.
Highlights
Gastric cancer is one of the most common cancers in the world
Tissue factor (TF) is higher in a variety of tumors, and TF-FVIIa complex plays an important role in tumor cell growth, invasion, angiogenesis, as well as metastasis, through a variety of signal transduction pathways (Rao and Pendurthi, 2005; Forster et al, 2006)
Human gastric cancer cell line SGC7901, which was transfected successfully with TF-pcDNA3 recombinant and empty vector pcDNA3 incubated with oxaliplatin, was used to investigate the invasion and apoptosis in human gastric cancer
Summary
Gastric cancer is one of the most common cancers in the world. Many Asian countries, including Korea, China, and Japan, have very high incidences of gastric cancer. Oxaliplatin has been used widely in advanced gastric cancer, and shows great prospect (Raymond et al, 1998). TF is higher in a variety of tumors, and TF-FVIIa complex plays an important role in tumor cell growth, invasion, angiogenesis, as well as metastasis, through a variety of signal transduction pathways (Rao and Pendurthi, 2005; Forster et al, 2006). The effect of TF on antitumor efficacy of oxaliplatin in human gastric cancer has not been reported. Human gastric cancer cell line SGC7901, which was transfected successfully with TF-pcDNA3 recombinant and empty vector pcDNA3 incubated with oxaliplatin, was used to investigate the invasion and apoptosis in human gastric cancer. TF reduces oxaliplatin in human gastric cancer cell invasiveness’ in vitro inhibitory effect, and reduces oxaliplatin-induced apoptosis
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