Effect of timing of restricted feeding on sexual maturation in female yellowtail, Seriola quinqueradiata

Abstract

In aquaculture of species with a large body size such as tuna and yellowtail, a major problem still is that broodstock management for egg collection requires high feeding cost and labor. In order to reduce feeding costs, development of restricted feeding techniques without affecting the reproductive performance may be useful. To gain a better understanding of reproductive responses to food supply, we examined the effect of restricted feeding during the previtellogenic and vitellogenic phases on sexual maturation in female yellowtail, Seriola quinqueradiata. Restricted feeding during the vitellogenic phase tended to reduce ovary weight relative to normally fed controls at the end of the experiment. Histological observations revealed that both restricted feeding regimes do not affect the percentage of atretic follicles as compared to the control group. Nevertheless, the mean diameter of the most advanced follicles of the females subjected to restricted feeding during the vitellogenic phase was significantly smaller to normally fed controls. These results suggest that reduced food intake during the vitellogenic phase is likely to delay oocyte growth, and lead to low ovarian weight. Endocrine analyses showed that restricted feeding during the vitellogenic phase reduced plasma 17β-estradiol levels, but not plasma and pituitary gene expression levels of gonadotropins (follicle-stimulating hormone and luteinizing hormone) at the end of the experiment. Transcripts for ovarian steroidogenesis-related genes, such as P450 side-chain cleavage (cyp11a1) and 3β-hydroxysteroid dehydrogenase (hsd3b) after food restriction during the vitellogenic phase tended to be high. Therefore, the effects of restricted feeding on reproduction would not be mediated by alterations in circulating gonadotropin levels, but rather through direct effects on the ovarian steroid production in yellowtail females.