Abstract
This study aims to determine the factors affecting the process of extraction of phenolic compounds from olive leaves. Two methods of extraction were used in this work and different tests were implemented with the aim of optimizing the recovery of phenolic compounds from olive leaves. The factors considered were the extraction time, temperature, solvent-solid ratio and the ethanol concentration. Obtained results show that the recommended optimal conditions for the extraction of the total phenolic compounds from olive leaves were found at 40°C with a solvent/solid ratio of 30:1 and ethanol concentration of 80% (v/v) when the dried olive leaves are stored at -21°C at the beginning of the experiments.
Highlights
The olive leaves have been used in traditional medicine in some Mediterranean regions
The recovery of phenolic compounds from olive leaves could be achieved by solvent extraction
It was found that higher total phenolic content is obtained by increasing temperature and solvent to solid ratio
Summary
The olive leaves have been used in traditional medicine in some Mediterranean regions. Utilizations of olive leaves to produce health promoting products and food ingredients have great potential for pharmaceutical and food industries. There are many methods for the extraction of antioxidants from olive leaves, and solvent extraction is the most common one. The solvents frequently used for the extraction of phenolic compounds and derivatives from olive leaves are methanol or hexane and water mixtures. It is aimed to define the optimum extraction conditions for phenolic compounds from dried olive leaves which allows the evaluation of different process variables such as solvent composition, temperature, time and solvent to solid ratio effects on total phenolic compound content in the extract
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