Effect of Time Fermentation and Saccharomyces Cerevisiae Concentration for Bioethanol Production from Empty Fruit Bunch

Abstract

Bioethanol is new renewable energy using the fermentation process from a substrate containing glucose. One of the materials which can convert to bioethanol is empty fruit bunch, which is in large amount in Indonesia. The plantation of palm oil has empty fruit bunch waste as much 23.988.293 tons in a year. The conversion from an empty fruit bunch to bioethanol also helps to reuse the waste which is not useful. There are a few steps to get bioethanol from an empty fruit bunch that is delignification of empty fruit bunch pollen using KOH solution which makes from empty fruit bunch ash getting from an incinerator. And then purification of pollen using H2O2 3% solution, hydrolysis cellulose becomes glucose solution using H2SO4 1% in process condition 100 °C and 60 minutes. Fermentation using temperature condition 30 °C, and stirring in 250 rpm. The variation of yeast Saccharomyces cerevisiae concentration which is 4 g/L, 6 g/L, and 8 g/L. And time variation in 0, 24, 48, 72, 96, and 120 hours. The research result is shown that maximum glucose from hydrolysis is 112,44 g/L. And the highest bioethanol concentration from the process is 5,5% (v/v) or 41,411 g/L in yeast concentration 6 g/L and 96 hours.