Effect of time and temperature of pretreatment and anaerobic co-digestion of rice straw and swine wastewater by domesticated paddy soil microbes

Abstract

Rice straw and swine wastewater are abundant, easy to obtain, and inexpensive biomass materials. Anaerobic digestion of rice straw and swine wastewater effectively regulates the carbon-to-nitrogen ratio and also improves methane production efficiency. The dense lignocellulosic structure, unsuitable carbon-to-nitrogen ratio, and light texture of rice straw hinder its application in anaerobic digestion. Effective pretreatment technologies can improve degradation efficiency and methane production. Our study is the first to apply domesticated paddy soil microbes to enhance the efficiency of hydrolytic acidification of rice straw and swine wastewater at varying temperatures and times. The results show that the highest total organic carbon (1757.2 mg/L), soluble chemical oxygen demand (5341.7 mg/L), and organic acid concentration (4134.6 mg/L) appeared in the hydrolysate after five days of hydrolytic acidification at 37 °C. Moreover, the use of hydrolysate produced 13% more gas and reduced the anaerobic digestion period by ten days compared to the untreated control. This suggests that using domesticated paddy soil microbes as a pretreatment might be a sustainable and cost-effective strategy for improving the degradation efficacy and methane production from lignocellulosic materials.