Effect of Thymus vulgaris leaves Extract and Ceftriaxone on induced Pyometra in mice

Abstract

Aim of study: The current study was made to estimate the role of Thymus vulgaris extract and ceftriaxone on induced pyometra. Materials and methods: 17a-ethinyl estradiol (15µg/kg) was used to induce pyometra. Fifty female animals were dispersed into five collections. The 1st group (G1) was induced and treated with a daily dose of Thymus vulgaris leaves extract (500 mg / kg) administered orally, the 2ed group (G2) was induced and given I/M daily dose of Ceftriaxone (5mg/kg), the 3ed group (G3) was induced and treated daily with Thymus vulgaris leaves extract (250 mg / kg) and given I/M daily dose of Ceftriaxone (2.5 mg/kg), while 4rth group (G4) was induced and treated with distilled water, with the 5rth group (G5) was without any treatment. Results: The PCV and Hb levels in G3 revealed a significant elevation as equated with G2 and G4. The values of WBCs, N% and M% in G1 and G3 indicated a significant elevation as equated with G2 and G5. The levels of albumin, globulin, FSH and LH in G1 and G3 exhibited a significant rise as equated with G2. Fertility index% in G3 revealed an improvement of fertility more than other treated groups. The histopathological section of the uterus in G4 displayed severe hemorrhage with hyperplasia of epithelial cells. The uterus in G1 exposed mononuclear cells penetration in all layers of the uterus. The uterus in G2 showed marked neutrophils and mononuclear cells penetration in subepithelial. The uterus section of the animal in G3 showed mononuclear cells infiltration in all layers of the uterus. Conclusion: From this study, we concluded that the using of Thymus vulgaris extract with ceftriaxone had the ability in treatment pyometra with reducing and preventing side effect of ceftriaxone.