Effect of three compounds extracted from Aiisma Orientalis ( Sam. ) juzep, on urinary calcium oxalate stones formation

Abstract

Objective To study the effect of three compounds extracted from Alisma Orientalis on urinary calcium oxalate stones formation and identify the effective constituents.Methods Three com-pounds were extracted from Alisma Orientalis.In the in vivo experiment, 50 adult healthy male Wistar strain rats,weighing 180-220 g,were randomly divided into 5 groups (n=10 each) :group A (normal con-trol group), group B (oxalate calcium stone formation group), group C (compound Ⅰ-treated group), group D (compound Ⅱ -treated group),group D (compound Ⅲ-treated group).After feeding for 4 weeks under the same conditions,24 h urine was collected and the levels of 24-h OX, and Ca2+ were determined.All kidneys were resected,and crystal/stone location, renal tubular dilation and the total of Ca2+ contents in the renal tissues of all rats were examined.Results The result of the in vivo experiment showed that in group C, renal tissue calcium content (10.35±6.45) and crystals deposition were significantly lower than those in group B except for 24 h urinary Ox excretion,but the 24-h urinary calcium excretion (61.49±2.06) was increased.Conclusion The compound I extracted from Alisma Orientalis seems to decrease renal tissue calcium content, tubular dilatation and crystals deposition but increase 24-h urinary calcium excretion in renal tissues in vivo, therefore to be the effective constituent of Alisma Orientalis to prevent calcium oxalate stone formation. Key words: Urinary calculi; Oriental Waterplantain Rhizome; Calcium oxalate; Pharmacology