Effect of “three-acupoint and five-needling method” on the expression level of p-ERK 1/2 protein in lung tissue of rats with allergic bronchial asthma

Abstract

ObjectiveTo explore the effect of “three-acupoint and five-needling method” on the expression level of p-ERK 1/2 protein in lung tissue of rats with allergic bronchial asthma. MethodsForty SPF-grade male SD rats were selected and randomly divided into four groups, including control group (group C), model group (group M), acupuncture group (group A) and blocker group (group PD), with 10 rats in each group. After corresponding intervention was given, cell counting was conducted, and the expression level of p-ERK 1/2 protein in the lung tissue of rats was detected via immunohistochemical method and Western blot method. ResultsThe number of leukocyte and eosinophil in the bronchoalveolar lavage fluid of rats in group A and group PD was smaller than that in group M, but larger than that in group C. It was shown based on the immunohistochemical test and Western blot test that the expression level of p-ERK1/2 protein in the lung tissue of rats in group M was higher than that in group C (P<0.05), while the expression level of p-ERK1/2 protein in group A and group PD was lower than that in group M (P<0.05). Conclusion“Three-acupoint and five-needling method” can effectively relieve the inflammatory response of rats with allergic bronchial asthma, and inhibit the expression level of p-ERK 1/2 protein in the lung tissue of rats with asthma. Acupuncture may alleviate the symptom of allergic bronchial asthma through inhibiting the expression level of p-ERK 1/2 protein.