Abstract
An inactive polypyrrole uricase electrode can be activated by thiourea and reused for determining the concentration of uric acid. We have observed an interesting reaction in which the oxidation of thiourea is catalysed by the uricase immobilized on a polypyrrole film in the absence of uric acid. The reaction rate of the enzyme electrode with respect to thiourea decreases with increasing pH value and increases with increasing applied potential and temperature. The activation energy of the enzyme-catalysed reaction for the thiourea is 42.2 kJ mol −1. The response current of the enzyme electrode in the absence of uric acid increases linearly with increasing concentration of thiourea in the range below 2.0 mmol dm −3. Thus the polypyrrole uricase electrode can be used to determine the concentration of thiourea.
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