Effect of thermal manipulation during Broiler chicken embryogenesis on the differential expression of Heat shock protein and Factor during heat stress on post‐hatch chicken

Abstract

The objective of this study was to determine the effect of thermal manipulation during broiler chicken embryogenesis on the differential expression of heat shock proteins (HSPs) and heat shock factors (HSFs) during thermal stress at post hatch days 14 and 28 of age. Fertile chicken eggs (n=1500) were divided into five incubation treatment groups (300 each): control group was maintained at 37.8 °C 56% relative humidity (RH) throughout the incubation period; TM1 was subjected to TM at 38.5°C for 24 h and 65% RH daily during ED12–18; TM2 was subjected to TM at 39°C for 24 h and 65% RH daily during ED12–18; TM3 was subjected to TM at 39.5°C for 24 h and 65% RH daily during ED12–18 and TM4 was subjected to TM at 40°C for 24 h and 65% RH daily during ED12–18. At post‐hatch days 14 and 28 of age, chicks in TC groups were subjected to thermal challenge (TC) at 41.0°C for 6 h while naïve chicks were kept under regular conditions. Chick's response to TC was evaluated by determination of body temperature (Tb) and differential muscle mRNA expression of Hsp108, Hsp90, Hsp70 and Hsp60. During TC at days14 and 28 of age, the Tb of TM chicks was significantly lower than those of the controls. When compared to the control, TM resulted in a rapid significant increase in mRNA levels of Hsps and HSFs in the muscle during TC at post hatch days 14 and 28 of age.Support or Funding InformationDeanship of Research, Jordan University of Science and Technology (Project ID# 66/2016).