Abstract
Flagella expression in Escherichia coli is controlled in a hierarchical manner, in which class-1 gene products, FlhDC, functions as a master regulator to control class-2 genes that encode motility-related genes. fliA, one of the class 2 genes, encodes flagellum-specific sigma factor (FliA/Sigma F/Sigma-28), which is necessary for the expression of class-3 genes. Previously, we carried out transcriptome analyses of all two-component regulatory systems of E. coli, and determined that the arcA mutant showed the motility-defective phenotype. In this study, we characterized the arcA mutant, and we present evidence that ArcA is necessary for the expression of FliA, but not for the master regulators, FlhDC. The phosphorylation site of ArcA is necessary for motility, while a cognate histidine kinase, ArcB, appears not to be involved in motility. This suggests that there must be regulatory factors other than ArcB interacting with ArcA to control flagella genes.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
