Effect of the Zn-aminoacid MOF concentration on the in vitro biocompatibility of red and green tomato seed cells over collagen-starch-ZnMOFs hydrogels

Abstract

A balance between macronutrients (N, P, K, Ca, Mg) and micronutrients (Fe, Zn, Mn, Mo, B, Cl) is essential for the thriving of red tomato (Solanum lycopersicum) and green tomato (Physalis philadelphica) plants as well as good water irrigation. Zn is a vital micronutrient in seed development, fruit set, and maturity, while histidine plays a critical role in plant growth and development. Hence, a MOF based on Zn and histidine (ZnHis) was added in different amounts (X=0, 10, 30, and 50 wt. %) into a composite hydrogel of collagen and starch to supply of water and micronutrients necessary for the in vitro proliferation, and cell migration of red tomato and green tomato cells living over these hydrogels. The functional groups and reticulation degree were assessed by ATR-FTIR and the ninhydrin assay. The metabolic activity of red tomato and green tomato cells living on these hydrogels was tested by reaction with MTT. Cell migration of red tomato and green tomato cells was studied by light microscopy. The results evidencing that 10 wt. % and 30 wt.% of ZnHis are the preferable concentrations to promote cell metabolism, and cell migration of red tomato and green tomato cells extracted from seeds