Abstract
Preparations of membrane-bounded systems including red blood cells, mitochondria, and microsomes were prepared for electron microscopy in the presence of ammonium molybdate, potassium phosphotungstate and lithium tungstate solutions at final concentrations of 1%. The effects of various negative stain pH levels on the specimens were also observed. The experiments have shown that membrane organization is well preserved in the presence of ammonium molybdate without prior fixation, whereas other negative stains were observed to damage membrane systems by hypotonic disruption unless previous fixation was applied. Concentrations of ammonium molybdate were compared with corresponding concentrations of sucrose most frequently used for cell fraction.
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