Abstract
Human fibroblasts(HFB) is responsible for treating hypertrophic scars due to their proliferation and the corresponding collagen secretion,which are influenced by human keratinocytes cells(HKC).This influence was investigated in the present work.HFB were cultured in a mixed solution with 50% HKC supernatant and 50% DMEM.This cultivation process was performed under 3.4 kPa pressure within 5 and10 h,respectively.In addition,the cultivation without pressure was settled as the control group.MTT method was used to determine the proliferation of HFB,while coloricmetric method was utilized to identify the collagen expression in the supernatant of HFB.The experimental results revealed that HFB proliferation and collagen were increased significantly in the supernatant of HKC groups(P0.05) with and without pressure.Compared to the control group,reduction of HFB proliferation and collagen significantly happened in the groups with 3.4 kPa pressure(P0.05).However,in the HKC group under 3.4 kPa pressure for 10 h,HFB proliferation was improved significantly(P0.05) but collagen was decreased significantly(P0.05).This investigation suggested that the supernatant of HKC played an important role in improving HFB proliferation and collagen,which was inhibited by 3.4 kPa pressure.
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