Abstract
BackgroundDue to the detrimental effect of blood contamination on the physico-chemical properties of mineral trioxide aggregate (MTA), obtaining an effective hemostasis in the surgical crypt during apical surgery is of paramount importance. The purpose of this in vivo study was to analyze the effect of Ankaferd Blood Stopper® (ABS) contamination on the biocompatibility of MTA.MethodsForty of 56 Wistar–Albino rats were divided randomly and equally into two groups (MTA and MTA-ABS) according to whether or not a hemostatic agent was used. The remaining 16 rats were designated as the control group. Rats in the experimental groups received freshly mixed MTA-Angelus in polyethylene tubes, which were inserted into monocortical bore holes created in their tibias. In the MTA-ABS group only, 0.5 mL of ABS solution was administered topically on the defect sites followed by implantation of MTA tubes. Inflammation, foreign-body reaction (FBR), necrosis, fibrosis, and new bone formation (NBF) were studied 7, 30, 60, and 90 days after implantation.ResultsOn day7, statistically significant differences were found in tissue reactions with regard to NBF and necrosis (p = 0.044 and p = 0.024, respectively), the latter being observed in 40 % of the samples only in the MTA-ABS group. Slight inflammation in all groups was confined to day-7 only. Mild necrosis was present in the MTA-ABS group only on day-7. Severity of the foreign body reaction and fibrosis was limited. New bone formation increased gradually over time in all groups, reaching a maximum on day-90.ConclusionsMTA and ABS-contaminated MTA are equally biocompatible. ABS does not impair the properties of MTA.Electronic supplementary materialThe online version of this article (doi:10.1186/s12903-016-0302-0) contains supplementary material, which is available to authorized users.
Highlights
Due to the detrimental effect of blood contamination on the physico-chemical properties of mineral trioxide aggregate (MTA), obtaining an effective hemostasis in the surgical crypt during apical surgery is of paramount importance
MTA is employed in these conditions because of its biocompatibility, antibacterial nature, ability to set in moist environments, and because it acts as a potential seal against bacterial penetration [8]
Scores and percentages for inflammation, foreign-body reaction (FBR), necrosis, fibrosis, and new bone formation (NBF) in all groups are shown in Tables 1–2 and illustrated in Fig. 1
Summary
Due to the detrimental effect of blood contamination on the physico-chemical properties of mineral trioxide aggregate (MTA), obtaining an effective hemostasis in the surgical crypt during apical surgery is of paramount importance. An “ideal” root-end filling material should have properties like; easy handling, good biocompatibility and apical sealability at the root apex to prevent the egress of bacteria, and bacterial byproducts [2, 3]. It should be insoluble in body fluids such as blood, Various root-end filling materials have been suggested [6]. MTA is employed in these conditions because of its biocompatibility, antibacterial nature, ability to set in moist environments, and because it acts as a potential seal against bacterial penetration [8]
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