Abstract
Background and Aim:Studies have shown that the pH of the vagina during the course of fertilization may influence the migration of X- and Y-bearing spermatozoa and thus leading to skewness in the sex of the offspring. Hence, this study was carried out to check the effect of the pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine (Bos indicus).Materials and Methods:To check the effect of pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine, we used buffers of various pH ranging from 5.5 to 9.0 for swim-up procedure of sperm sample and collected upper and bottom fraction from the same buffer and checked the abundance of X- and Y-bearing spermatozoa by droplet digital polymerase chain reaction using X- and Y-chromosome-specific DNA probe.Results:The abundance of X- and Y-bearing spermatozoa was not differed significantly in either of the fraction collected.Conclusion:Thus, it appears to be unlikely that an immediate impact of pH on sperm can be a solitary impact on the sex of offspring in bovine.
Highlights
Various scientists have been researching if the natural framework deciding the sex of an infant is totally arbitrary or can be pre-selected
Conclusion: it appears to be unlikely that an immediate impact of pH on sperm can be a solitary impact on the sex of offspring in bovine
When Ringer’s lactate solution was used for preparing buffer of different pH from 5 to 10, sperm was motile from pH 5.5, 6, 7, and 8 only, whereas when Dulbecco’s Modified Eagle Medium (DMEM) medium was used for preparing buffer of different pH from 5 to 10, sperm was motile from pH 5.5, 6, 7, 8, and 9
Summary
Various scientists have been researching if the natural framework deciding the sex of an infant is totally arbitrary or can be pre-selected. Despite the knowledge regarding differences between X and Y spermatozoa (for example, head measure [3], motile speed [4,5], DNA content [6,7], and pH susceptibility [8]) is available, still the definite physiological reasons for skewness in the proportion of spermatozoa carrying X or Y chromosomes with respect to environmental factors are not clear [9]. The only commercially available and effective method is using the difference in DNA content in the X- and Y-bearing spermatozoa [10,11]. Other methods where studies were carried out depending on the slight difference in density of X- and Y-bearing spermatozoa [12], Percoll and albumin gradient [13,14,15], Copyright: Raval, et al Open Access. This study was carried out to check the effect of the pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine (Bos indicus)
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