Abstract
Heterosis is a common biological phenomenon that can be used to optimize yield and quality of crops. Using heterosis breeding, hybrids with suitable nicotine content have been applied to tobacco leaf production. However, the molecular mechanism of the formation of nicotine heterosis has never been explained from the perspective of protein. The DIA proteomics technique was used to compare the differential proteomics of the hybrid Va116 × Basma, showing strong heterosis in nicotine content from its parent lines Va116 and Basma. Proteomics analysis indicated that 65.2% of DEPs showed over-dominant expression patterns, and these DEPs included QS, BBL, GS, ARAF and RFC1 which related to nicotine synthesis. In addition, some DEPs (including GST, ABCE2 and ABCF1 and SLY1) that may be associated with nicotinic transport exhibited significant heterosis over the parental lines. These findings demonstrated that the efficiency of the synthesis and transport of nicotine in hybrids was significantly higher than that in the parent lines, and the accumulation of over-dominant expression proteins may be the cause of heterosis of nicotinic content in hybrids.
Highlights
Heterosis is a common biological phenomenon that can be used to optimize yield and quality of crops
Heterosis genes related to rice tillering have been polymerized by Graded Pool-Seq and integrated genomic methods, and it has been reported that the dominance or incomplete dominance of heterozygous loci in rice plays an important role in the formation of h eterosis[8,9,10]
In order to explore the heterosis of nicotine content in the leaves of tobacco hybrids, 11 materials with different nicotine contents were selected as parents and matched with hybrid combinations
Summary
Heterosis is a common biological phenomenon that can be used to optimize yield and quality of crops. The molecular mechanism of the formation of nicotine heterosis has never been explained from the perspective of protein. Some DEPs (including GST, ABCE2 and ABCF1 and SLY1) that may be associated with nicotinic transport exhibited significant heterosis over the parental lines. These findings demonstrated that the efficiency of the synthesis and transport of nicotine in hybrids was significantly higher than that in the parent lines, and the accumulation of over-dominant expression proteins may be the cause of heterosis of nicotinic content in hybrids. For the formation of the pyridine ring, QPT is the rate-limiting enzyme for the synthesis and metabolism of n icotine[24]. About 80% of the characteristics in the first generation of flue-cured tobacco hybrids showed different degrees of h eterosis[28], with the heterosis
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