Effect of the natural state of an elastic cellular membrane on tank-treading and tumbling motions of a single red blood cell

Abstract

A two-dimensional computer simulation model was proposed for tank-treading and tumbling motions of an elastic biconcave red blood cell (RBC) under steady shear flow. The RBC model consisted of an outer cellular membrane and an inner fluid; the membrane's elastic properties were modeled by springs for stretch/compression and bending to consider the membrane's natural state in a practical manner. Membrane deformation was coupled with incompressible viscous flow of the inner and outer fluids of the RBC using a particle method. The proposed simulation model was capable of reproducing tank-treading and tumbling motions of an RBC along with rotational oscillation, which is the transition between the two motions. In simulations using the same initial RBC shape with different natural states of the RBC membrane, only tank-treading motion was exhibited in the case of a uniform natural state of the membrane, and a nonuniform natural state was necessary to generate the rotational oscillation and tumbling motion. Simulation results corresponded to published data from experimental and computational studies. In the range of simulation parameters considered, the relative membrane elastic force versus fluid viscous force was approximately 1 at the transition when the natural state nonuniformity was taken into account in estimating the membrane elastic force. A combination of natural state nonuniformity and elastic spring constant determined that change in the RBC deformation at the transition is that from a large compressive deformation to no deformation, such as rigid body.